miR-133a Enhances the Protective Capacity of Cardiac Progenitors Cells after Myocardial Infarction
miR-133a Enhances the Protective Capacity of Cardiac Progenitors Cells after Myocardial Infarction
Blog Article
miR-133a and miR-1 are known as muscle-specific microRNAs that are involved in cardiac development and pathophysiology.We have shown that both miR-1 and miR-133a are early and progressively upregulated during in vitro cardiac differentiation of adult cardiac progenitor cells (CPCs), but only miR-133a expression was enhanced under in vitro oxidative stress.miR-1 was demonstrated to favor differentiation of CPCs, whereas miR-133a Ice Maker Accessories overexpression protected CPCs against cell death, targeting, among others, the proapoptotic genes Bim and Bmf.
miR-133a-CPCs clearly improved cardiac function in a rat myocardial infarction model by reducing fibrosis and hypertrophy and increasing vascularization and cardiomyocyte proliferation.The beneficial effects of miR-133a-CPCs Wood Pipe seem to correlate with the upregulated expression of several relevant paracrine factors and the plausible cooperative secretion of miR-133a via exosomal transport.Finally, an in vitro heart muscle model confirmed the antiapoptotic effects of miR-133a-CPCs, favoring the structuration and contractile functionality of the artificial tissue.